One-year clinical efficacy and safety of indigo naturalis for active ulcerative colitis: a real-world prospective study

Yuichi Matsuno; Takehiro Torisu; Junji Umeno; Hiroki Shibata; Atsushi Hirano; Yuta Fuyuno; Yasuharu Okamoto; Shin Fujioka; Keisuke Kawasaki; Tomohiko Moriyama; Tomohiro Nagasue; Keizo Zeze; Yoichiro Hirakawa; Shinichiro Kawatoko; Yutaka Koga; Yoshinao Oda; Motohiro Esaki; Takanari Kitazono

doi:10.5217/ir.2021.00124

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Original Article One-year clinical efficacy and safety of indigo naturalis for active ulcerative colitis: a real-world prospective study: Yuichi Matsuno¹, Takehiro Torisu¹, Junji Umeno¹, Hiroki Shibata², Atsushi Hirano¹, Yuta Fuyuno¹, Yasuharu Okamoto¹, Shin Fujioka¹, Keisuke Kawasaki¹, Tomohiko Moriyama^1,3, Tomohiro Nagasue¹, Keizo Zeze¹, Yoichiro Hirakawa^1,4, Shinichiro Kawatoko⁵, Yutaka Koga⁵, Yoshinao Oda⁵, Motohiro Esaki⁶, Takanari Kitazono¹; Intestinal Research 2022;20(2):260-268.
DOI: https://doi.org/10.5217/ir.2021.00124
Published online: April 29, 2022

¹Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Fukuoka, Japan

²Division of Genomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan

³International Medical Department, Kyushu University Hospital, Fukuoka, Japan

⁴Department of Epidemiology and Public Health, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

⁵Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

⁶Division of Gastroenterology, Department of Internal Medicine, Saga University, Saga, Japan

Correspondence to Takehiro Torisu, Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Tel: +81-92-642-5261, Fax: +81-92-642-5273, E-mail: torisu.takehiro.437@m.kyushu-u.ac.jp

• Received: September 10, 2021 • Revised: November 1, 2021 • Accepted: November 8, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Abstract
INTRODUCTION
METHODS
RESULTS
DISCUSSION
NOTES
Supplementary Material
REFERENCES

Abstract

Background/Aims
Recent studies suggested a favorable effect of indigo naturalis (IN) in inducing remission for refractory ulcerative colitis (UC), however, the maintenance effect of IN for patients with UC remains unknown. Therefore, we conducted a prospective uncontrolled open-label study to analyze the efficacy and safety of IN for patients with UC.
Methods
Patients with moderate to severe active UC (clinical activity index [CAI] ≥ 8) took 2 g/day of IN for 52 weeks. CAI at weeks 0, 4, 8, and 52 and Mayo endoscopic subscore (MES) and Geboes score (GS) at weeks 0, 4, and 52 were assessed. Clinical remission (CAI ≤ 4), mucosal healing (MES ≤ 1), and histological healing (GS ≤ 1) rates at each assessment were evaluated. Overall adverse events (AEs) during study period were also evaluated. The impact of IN on mucosal microbial composition was assessed using 16S ribosomal RNA gene sequences.
Results
Thirty-three patients were enrolled. The rates of clinical remission at weeks 4, 8, and 52 were 67%, 76%, and 73%, respectively. The rates of mucosal healing at weeks 4 and 52 were 48% and 70%, respectively. AEs occurred in 17 patients (51.5%) during follow-up. Four patients (12.1%) showed severe AEs, among whom 3 manifested acute colitis. No significant alteration in the mucosal microbial composition was observed with IN treatment.
Conclusions
One-year treatment of moderate to severe UC with IN was effective. IN might be a promising therapeutic option for maintaining remission in UC, although the relatively high rate of AEs should be considered.
Keywords: Indigo naturalis; Qing-Dai; Colitis, ulcerative; Maintenance therapy

INTRODUCTION

Ulcerative colitis (UC) is a chronic inflammatory condition of the colorectum that is characterized by relapsing and remitting cycles [1]. Medical treatment of UC is divided into induction and maintenance therapies. While the main target of induction therapy is achieving mucosal healing, the ultimate goal in patients with refractory UC is to avoid surgery. However, more than 20% of patients with UC are obliged to undergo total colectomy during their clinical course [2]. Despite the recent increase in treatment options for refractory UC, most of these medications cause immunosuppression, raising concerns about the increased risk of serious infections, especially in older patients. Thus, additional therapeutic options with a new mode of action and minimal safety concerns are warranted for better clinical outcomes in refractory UC.

Indigo naturalis (IN), also referred to as Qing-Dai, is extracted from the leaves and stems of plants, including Indigofera tinctoria and Strobilanthes cusia. Indigo and indirubin molecules, which are the main components of IN, were reported to be endogenous human aryl hydrocarbon receptor (AhR) ligands [3]. AhR signaling was reported to improve colitis in mice via interleukin-22 (IL-22) [4] by promoting mucosal healing [5]. IN also ameliorated murine DSS-induced colitis by upregulating IL-10 and IL-22 via AhR activation [6]. The regulation of host-microbiota communication was suggested to be a potential mechanism related to mucosal healing by AhR signaling [7,8].

Several studies have demonstrated the favorable efficacy of IN to induce remission in UC [9,10], and IN was suggested to be the choice of induction therapy for such patients. Conversely, IN is not generally considered an herbal medicine for longterm use. However, our previous retrospective study suggested the possible maintenance effect of IN in refractory UC [11]. Therefore, we conducted this prospective study with different participants to determine the clinical efficacy and safety of IN as maintenance therapy for patients with UC. In this study, we also assessed the influence of IN maintenance therapy on mucosal microbiota using mucosal biopsy samples, on the basis that induction therapy by IN might modify the fecal microbiota composition [10].

METHODS

1. Study Entry Criteria

This prospective uncontrolled open-label study was conducted at Kyushu University Hospital. We recruited subjects with UC who fulfilled both the inclusion and exclusion criteria, as described below. The diagnosis of UC was confirmed by the criteria established by the Japanese Ministry of Health, Labour, and Welfare [12]. The inclusion criteria were as follows: (1) patients aged ≥ 16 years; (2) moderate to severe active UC (clinical activity index [CAI] ≥ 8 and Mayo endoscopic subscore [MES] ≥ 2); (3) left-sided or extensive UC; and (4) no history of IN treatment. The exclusion criteria were as follows: (1) pregnant or breastfeeding women; (2) patients with psychosomatic disorders, liver, or renal disease; and (3) patients with thrombocytopenia or taking antithrombotic therapy.

2. Study Protocol

Powdered IN was purchased from Seishin-shoyakudo (Tokyo, Japan). Each patient took 1 g of IN capsules twice a day (2 g/day orally). When adverse events (AEs) that were possibly related to IN occurred during the study period, the daily dose of IN was reduced as appropriate in the attending physicians’ discretion. No increase in the dosage of concurrent medications and no additional treatment for UC was allowed within 2 weeks before the study baseline. During the study period, concurrent medications could be reduced or stopped based in the attending physicians’ discretion. However, when the dosage of concurrent medications was increased or new medications were added, the patient was considered to have exacerbated UC.

The clinical disease activity was assessed by trained physicians (T.T., J.U., A.H., Y.F., Y.O., S.F., T.M., and M.E.) at weeks 0, 4, 8, and 52 using the CAI [13]. The endoscopic disease activity was assessed at weeks 0, 4, and 52 based on the MES [14] by experienced endoscopists who specialize in inflammatory bowel disease (Y.M., T.T., J.U., A.H., Y.F., Y.O., S.F., T.M., and M.E.). The histological disease activity was assessed using the Geboes score (GS) [15]. Each rectal biopsy sample was assessed by pathologists (S.K., Y.O., and Y.O.) at weeks 0, 4, and 52.

Medical interviews and blood tests were performed at each clinical visit, and the results were analyzed by trained physicians to determine AEs related to IN. In the middle of the study period, the possible association of IN with pulmonary arterial hypertension (PAH) [16] was suspected, and medical interviews concerning breathlessness and echocardiogram at weeks 0, 4, and 52 were added thereafter.

3. Data Collection and Definitions

Clinical information for each patient was determined at study entry and was classified based on the Montreal classification [17].

At each assessment, clinical response was defined as a decrease in the CAI score of at least 30% from baseline. Clinical remission was defined as CAI ≤ 4. Mucosal healing was defined as MES ≤ 1, and complete mucosal healing was defined as MES = 0. Histological healing was defined as GS ≤ 1.

AEs were classified as mild or severe. Severe types included AEs that required hospitalization, while mild types included those not requiring hospitalization. For blood test abnormalities, liver dysfunction was defined as a serum transaminase level that was greater than twice the upper normal limit. Renal dysfunction was defined as the decrease of the estimated glomerular filtration rate by ≥ 30% from the baseline level. We regarded colitis with markedly edematous changes that were recovered only by the discontinuation of IN as an AE.

4. Biopsy Sampling

Mucosal biopsy samples were collected at weeks 0 and 52 during colonoscopy. For each colonoscopy, patients were prepared using standard bowel cleaning with 2 L of lavage solution (Niflec^®; EA Pharma Co., Tokyo, Japan). Mucosal biopsy samples were obtained from the rectum using disposable biopsy forceps (Olympus, Tokyo, Japan) after washing the rectal mucosa gently with tap water containing a dimethicone solution. Biopsy samples were immediately frozen at −80°C until extraction of microbial DNA.

5. DNA Extraction, Sequencing, and Processing of 16S Ribosomal RNA Gene

Mucosal microbial DNA was extracted as previously described with minor modification [18,19]. The V4 region of the 16S ribosomal RNA gene was amplified by polymerase chain reaction using the primers 515F/806R, which included the Illumina flow cell adapter sequence and a unique 12-base barcode sequence. Subsequently, multiplexed 16S amplicon sequencing was performed on the MiSeq Illumina platform (Illumina Inc., San Diego, CA, USA). Detailed methods are described in the Supplementary Material. The sequences were analyzed using QIIME version 1.9.1 software [20], which is available at http://www.qiime.org.

6. Statistical Analysis

Categorical variables were described using frequencies and percentages, and continuous variables were described using medians and interquartile ranges (IQRs). Clinical response and remission rates were calculated using non-responder imputation, where dropouts were assumed to be non-responders regardless of their actual disease activity at the time of dropout. The mucosal healing and histological healing rates were calculated using non-responder imputation. Changes in CAI, MES, C-reactive protein (CRP), and GS were estimated by the Wilcoxon signed-rank test. P-values were considered to be statistically significant at a level of < 0.05.

7. Ethical Considerations

This study protocol was approved by the Institutional Review Board at Kyushu University in compliance with the Declaration of Helsinki Principles (registration number: 27067). All patients enrolled in this study received and reviewed the study information, and then voluntarily provided written informed consent upon participation. This study was registered at the University Hospital Medical Information Network Center (UMIN Clinical Trials Registry, number UMIN 000022575).

RESULTS

1. Patients’ Characteristics

We offered 36 patients, and 2 patients declined to participate, a patient did not meet the inclusion criteria. Thirty-three patients with UC were enrolled in the present study (Supplementary Fig. 1). The baseline characteristics of the patients are shown in Table 1. The median age was 29 years (IQR, 23–48 years) and the median disease duration was 3 years (IQR, 1–8 years). The left-sided type was present in 5 patients and the extensive type was present in 28 patients. The median CAI score, serum CRP level, and MES at baseline were 8 (IQR, 8–11), 3.0 mg/L (IQR, 1.4–8.9 mg/L), and 2 (IQR, 2–3), respectively.

The main reasons for the induction of IN were as follows. Four patients were steroid refractory. There were 12 steroid dependent patients, 10 of whom were on oral prednisolone (PSL) while tapering off and 2 were relapsed and entered this study after discontinuation of PSL. PSL was being administered to 14 patients at study enrolment. Primary non-responder to anti-tumor necrosis factor (TNF)-α agents were 7 patients, secondary failure to anti-TNF-α agents were 5 patients. These 12 patients had been continuously treated with anti-TNF-α agent therapy for more than 8 weeks prior to study enrolment. Two patients were relapsed after cessation of tacrolimus, and 1 patient had insufficient response to cyclosporine. There were 2 patients with no induction therapy other than IN. Concurrent medicines were shown in Table 1. No patient was newly started on a drug other than IN as induction therapy.

2. Clinical Efficacy of IN as Induction and Maintenance Therapies

The clinical response rates at weeks 4, 8, and 52 were 93.9% (31/33), 84.8% (28/33), and 72.7% (24/33), and the clinical remission rates at each assessment were 57.6% (19/33), 69.7% (23/33), and 72.7% (24/33), respectively (Fig. 1). The trends for CAI were described in Supplementary Fig. 2, and the details of the clinical courses were as follows. Among 33 patients, 2 were primary non-responders to IN and 1 discontinued IN because of an AE. Six patients discontinued IN during follow-up because of a loss of response to IN in 3, unwillingness to continue the study in 2, and AEs in 1 patient. The remaining 24 patients maintained clinical remission at week 52 (Supplementary Fig. 1). All of the 24 patients showed clinical response at week 4. Of the 24 patients, 79% (19/24) achieved clinical remission within 8 weeks and the remaining 21% after 8 weeks (Fig. 2).

Fig. 3 shows the trends for CAI and CRP scores. The median CAI score was significantly lower at weeks 4, 8, and 52 than before the administration of IN (P<0.001, P<0.001, P<0.001, respectively). The median CRP score was also significantly lower at weeks 4 and 52 than before the administration of IN (P= 0.001, P< 0.001, respectively).

Endoscopic assessment was performed in 32 patients at week 4 and 24 patients at week 52. The mucosal healing rates at each assessment were 51.5% (17/33) and 69.7% (23/33), respectively. The complete mucosal healing rates at weeks 4 and 52 were 15.2% (5/33) and 54.5% (18/33), respectively (Fig. 4A). The median MES was significantly lower at weeks 4 and 52 than before the administration of IN (P< 0.001, P< 0.001, respectively) (Fig. 4B). Histological disease activity was assessed in 28 patients at week 4 and 18 patients at week 52. The median GS was also significantly lower at weeks 4 and 52 than before the administration of IN (P= 0.014, P< 0.001, respectively) (Fig. 4C).

Among 14 patients with concurrent PSL at baseline, 8 patients were achieved clinical remission with IN therapy at week 52 and were able to discontinue PSL. Regarding other concurrent therapy, anti-TNF-α agents were discontinued in 5 (5/12, 41.7%) and thiopurine were stopped in 3 patients (3/18, 16.7%), and remission was maintained with IN treatment during follow-up period.

3. Safety Assessment

Twenty-four AEs were reported in 17 patients during the study period (Table 2). Among them, 19 AEs (headache, liver dysfunction, mild epigastric pain/abdominal pain, and renal dysfunction) in 15 patients were considered mild, and 7 AEs (acute colitis, intussusception, infectious colitis, portal thrombosis, and appendicitis) in 4 patients were considered severe.

All mild AEs except for renal dysfunction occurred relatively early after induction therapy with IN and were reversible. However, a dose reduction of IN was required to improve abdominal pain in 1 patient and liver dysfunction in 2 patients. The other patients continued IN at a dose of 2 g/day, and the AEs were resolved without additional therapeutic intervention. Renal dysfunction was found in 1 patient treated concurrently with an anti-TNF-α agent. The estimated glomerular filtration rate of this patient gradually decreased from 72.4 mL/min/1.73 m² at baseline to 49.6 mL/min/1.73 m² at week 52.

Severe AEs were reported in 4 patients. Acute colitis developed in 3 patients, of whom 1 developed complicated intussusception. Three patients manifested acute severe abdominal pain and computed tomography showed marked colonic wall edema, as previously described [21-23]. However, 1 patient developed acute colitis 8 months later, with complications that reportedly occurred within 3 months after the induction of IN [11]. Another patient subsequently developed acute appendicitis after the improvement of colitis and required surgery. The remaining patient with infectious colitis caused by Clostridium perfringens simultaneously developed portal vein thrombosis. No patient showed complications related to PAH during follow-up.

4. Comparison of Microbial Composition before and after IN Treatment

The microbial composition was compared before and after IN treatment in 21 of 24 patients who achieved clinical remission at week 52. Additionally, 1,616,663 reads were obtained after quality control with a mean 62,179 reads per sample. Eight patients were excluded from the analysis because the number of reads from their samples were too small ( < 5,000). Consequently, the differences in microbial composition before and after IN were compared in 13 patients. The alpha diversity by the observed species index was not significantly different before and after IN treatment (P= 0.154) (Fig. 5A). The beta diversity by the metrics of weighted UniFrac also did not differ significantly (P= 0.148) (Fig. 5B). We subsequently compared the taxonomic composition before and after IN treatment. The abundance of genera Ruminococcus, Dorea, Actinomyces, Oribacterium, Eubacterium, Clostridium, and Finegoldia, the family Caulobacteraceae, and the order Streptophyta were significantly greater, and the abundance of genus Pediococcus was significantly lower, in mucosal samples after IN compared with those before IN (Supplementary Fig. 3). However, no significant difference was observed in the abundance of the Bacteroides and Bifidobacterium families, in contrast to a previous report [10].

DISCUSSION

Although IN as induction therapy for UC has shown favorable clinical efficacy, its maintenance effect remains uncertain. As for the induction therapy, the present study also showed that the clinical response rates at 8 weeks were 84.8%. The subjects were all different from the participants in previous retrospective study [11], yet they showed similar efficacy at 8 weeks. Several studies also reported the usefulness of IN as induction therapy in active UC [9-11]. As for the maintenance therapy, our prospective study showed the clinical response rate and remission rate at week 52 was 72.7%, indicating the high efficacy of IN as long-term effect as well.

Regardless of the efficacy shown by IN, there are some safety concerns [9,10,24]. A recent Japanese nationwide survey reported that mild AEs related to IN included liver dysfunction, gastrointestinal symptoms, and headache, and these occurred in 4.6%, 2.4%, and 1.5% of patients, respectively [25]. Our study revealed that mild AEs related to IN occurred at a much higher rate. These mild AEs could be missed due to the degree of physician awareness, therefore, physicians should be aware that mild AEs occur more frequently than reported previously. Additionally, most mild AEs were resolved only when the IN dose was reduced.

In the present study, 7 severe AEs that require hospitalization occurred in 4 patients, and acute colitis was the most frequent severe AE (9%). Based on the Japanese nationwide survey, the risk of colonic intussusception and colitis were 1.1% and 0.9%, respectively [25]. Acute colitis caused by IN was characterized by the acute onset of colicky abdominal pain and endoscopically severe edematous mucosa without mucosal defects, which is distinct from flare-up of UC [21-23]. This complication was reported to occur within 8 weeks after induction therapy with IN, and occasionally develops into colonic intussusception [21,23]. Although portal vein thrombosis was founded in the present study, dehydration caused by infectious colitis also occurred, which may be indirect AE of IN. The most alarming AE reported in the past was PAH [25]. PAH was not observed in the present study, probably because PAH was rare AE. It is controversial whether PAH caused by IN can recover after the discontinuation of IN or not [26,27], therefore, physicians should carefully consider PAH during the administration of IN.

The optimal IN dose to induce remission remains undetermined. A recent randomized controlled trial (RCT) showed a significant dose-dependent linear trend in the clinical response at week 8, but 2.0 g/day of IN had no advantage for clinical remission and mucosal healing compared with 1.0 g/day of IN [10]. Because the results of the RCT had not been published at the beginning of the present study, we started IN at a dose of 2.0 g/day as induction therapy. Considering that most AEs were resolved after dose reduction in the present study, optimal dose with further reduction needs to be clarified.

In the present study, the possible influence of IN on colonic microbial composition was assessed using mucosal biopsy samples at 52 weeks. AhR signaling, the main mechanistic pathway that mediates the effects of IN, was reported to modulate host-microbiota communication [7,8]. Additionally, Naganuma et al. [10] demonstrated that IN might partly modify colonic microbiota composition using fecal samples at 8 weeks. However, we did not find a significant influence of IN on colonic microbial diversity. The modifying effect of IN on colonic microbial diversity plays may change with long-term time.

Our study had some limitations. First, this study was an uncontrolled open-label study without a placebo group. Because a high placebo effect in UC is well known,28 an RCT will provide more reliable results to determine the true clinical efficacy of IN. Second, this study was performed at a single center with a limited number of patients. However, we think that the present study will have some clinical impact because this was the first prospective study to analyze the efficacy of IN in maintaining remission in patients with UC.

In conclusion, IN might be a therapeutic option to induce and maintain remission in moderate to severe UC, although the relatively high rate of AEs should be considered. Further studies are required to determine the appropriate dose to induce and maintain remission and to evaluate the safety concerns related to IN for refractory UC.

NOTES

Funding Source

The authors received no financial support for the research, authorship, and/or publication of this article.

Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Author Contribution

Conceptualization: Matsuno Y, Hirano A, Fuyuno Y, Esaki M. Data curation: Matsuno Y, Hirano A. Formal analysis: Matsuno Y, Hirano A, Hirakawa Y. Investigation: Matsuno Y, Umeno J, Shibata H, Hirano A, Fuyuno Y, Okamoto Y, Fujioka S, Kawasaki K, Moriyama T, Nagasue T, Zeze K, Kawatoko S, Koga Y, Oda Y, Esaki M. Methodology: Matsuno Y, Shibata H, Hirano A. Supervision: Torisu T, Umeno J. Writing - original draft: Matsuno Y. Writing - review & editing: Torisu T, Hirano A, Esaki M, Kitazono T. Approval of final manuscript: all authors.

Others

We thank the patients for participating in this study.

Supplementary Material

Supplementary materials are available at the Intestinal Research website (https://www.irjournal.org).

Supplementary Material

ir-2021-00124-suppl1.pdf

Supplementary Fig. 1. Study flowchart.

ir-2021-00124-suppl2.pdf

Supplementary Fig. 2. Individual clinical activity index scores at weeks 0, 4, 8, and 52 after the initiation of indigo naturalis.

ir-2021-00124-suppl3.pdf

Supplementary Fig. 3. Comparison of the taxonomic composition between before and after indigo naturalis indigo naturalis (IN) treatment. The abundance of genera Ruminococcus, Dorea, Actinomyces, Oribacterium, Eubacterium, Clostridium, and Finegoldia, the family Caulobacteraceae, and the order Streptophyta were significantly greater, and the abundance of genus Pediococcus was significantly lower, in mucosal samples after IN treatment compared with before IN treatment. LDA, linear discriminant analysis.

ir-2021-00124-suppl4.pdf

Fig. 1.

The clinical response rate and clinical remission rate at weeks 4, 8, and 52 after initiation of indigo naturalis were calculated using non-responder imputation.

Fig. 2.

Indicates the week in which the patients achieved a new clinical remission. Thirty patients achieved clinical remission. Although most patients respond early to indigo naturalis treatment, some had delayed response.

Fig. 3.

Trends for clinical activity index (CAI) and C-reactive protein (CRP) scores. (A) CAI scores at weeks 0, 4, 8, and 52 after the initiation of indigo naturalis (IN). (B) CRP levels at weeks 0, 4, and 52 after the initiation of IN.

Fig. 4.

(A) The mucosal healing rate and the complete mucosal healing rate at weeks 4 and 52 were calculated using non-responder imputation. Histological healing was defined as Geboes score ≤1. (B) Mayo endoscopic subscores at weeks 0, 4, and 52 after the initiation of indigo naturalis (IN). (C) Geboes scores at weeks 0, 4, and 8 after the initiation of IN.

Fig. 5.

(A) Alpha diversity by the observed species index and (B) beta diversity by the metrics of weighted UniFrac were not significantly different before and after indigo naturalis treatment. The lines in the box are median and whiskers are interquartile range.

Table 1.

Clinical Characteristics of UC Patients

Characteristics	Value (n = 33)
Age (yr)	29 (23–48)
Male sex	18 (53)
Disease duration (yr)	3 (1–8)
Extent of the disease
E2 (left-sided UC)	5 (15)
E3 (extensive UC)	28 (85)
Concurrent medications
Prednisolone	14 (42)
Dose of prednisolone (mg)	6.3 (5.0–18.8)
Anti-TNF-α agents	12 (36)
Cyclosporine	1 (3)
5-ASA	26 (79)
Thiopurine	18 (55)
CAI score	8 (8–11)
MES	2 (2–3)
CRP (mg/L)	3.0 (1.4–8.9)

Values are presented as median (interquartile range) or number (%).

UC, ulcerative colitis; TNF, tumor necrosis factor; 5-ASA, 5-aminosalicylic acid; CAI, clinical activity index (Rachmilewitz index); MES, Mayo endoscopic subscore; CRP, C-reactive protein.

Table 2.

Summary of Adverse Events

Adverse events	No. (%)	Duration after initiation of indigo naturalis (day)
Mild adverse events
Headache	8 (24)	1–14
Liver dysfunction	5 (15)	14–41
Mild epigastric pain/abdominal pain	5 (15)	1–28
Renal dysfunction	1 (3)	203
Severe adverse events
Acute colitis	3 (9)	25–258
Infectious colitis	1 (3)	49
Intussusception	1 (3)	25
Appendicitis	1 (3)	270
Portal vein thrombosis	1 (3)	55

REFERENCES

1. Langholz E, Munkholm P, Davidsen M, Binder V. Course of ulcerative colitis: analysis of changes in disease activity over years. Gastroenterology 1994;107:3–11.Article PubMed
2. Hoie O, Wolters FL, Riis L, et al. Low colectomy rates in ulcerative colitis in an unselected European cohort followed for 10 years. Gastroenterology 2007;132:507–515.Article PubMed
3. Adachi J, Mori Y, Matsui S, et al. Indirubin and indigo are potent aryl hydrocarbon receptor ligands present in human urine. J Biol Chem 2001;276:31475–1478.Article PubMed
4. Monteleone I, Rizzo A, Sarra M, et al. Aryl hydrocarbon receptor-induced signals up-regulate IL-22 production and inhibit inflammation in the gastrointestinal tract. Gastroenterology 2011;141:237–248.Article PubMed
5. Mizoguchi A, Yano A, Himuro H, Ezaki Y, Sadanaga T, Mizoguchi E. Clinical importance of IL-22 cascade in IBD. J Gastroenterol 2018;53:465–474.Article PubMed PMC PDF
6. Kawai S, Iijima H, Shinzaki S, et al. Indigo naturalis ameliorates murine dextran sodium sulfate-induced colitis via aryl hydrocarbon receptor activation. J Gastroenterol 2017;52:904–919.Article PubMed PDF
7. Qiu J, Guo X, Chen ZM, et al. Group 3 innate lymphoid cells inhibit T-cell-mediated intestinal inflammation through aryl hydrocarbon receptor signaling and regulation of microflora. Immunity 2013;39:386–399.Article PubMed PMC
8. Behnsen J, Raffatellu M. Keeping the peace: aryl hydrocarbon receptor signaling modulates the mucosal microbiota. Immunity 2013;39:206–207.Article PubMed
9. Suzuki H, Kaneko T, Mizokami Y, et al. Therapeutic efficacy of the Qing Dai in patients with intractable ulcerative colitis. World J Gastroenterol 2013;19:2718–2722.Article PubMed PMC
10. Naganuma M, Sugimoto S, Mitsuyama K, et al. Efficacy of indigo naturalis in a multicenter randomized controlled trial of patients with ulcerative colitis. Gastroenterology 2018;154:935–947.PubMed
11. Matsuno Y, Hirano A, Torisu T, et al. Short-term and long-term outcomes of indigo naturalis treatment for inflammatory bowel disease. J Gastroenterol Hepatol 2020;35:412–417.Article PubMed PDF
12. Matsuoka K, Kobayashi T, Ueno F, et al. Evidence-based clinical practice guidelines for inflammatory bowel disease. J Gastroenterol 2018;53:305–353.Article PubMed PMC PDF
13. Rachmilewitz D. Coated mesalazine (5-aminosalicylic acid) versus sulphasalazine in the treatment of active ulcerative colitis: a randomised trial. BMJ 1989;298:82–86.Article PubMed PMC
14. Schroeder KW, Tremaine WJ, Ilstrup DM. Coated oral 5-aminosalicylic acid therapy for mildly to moderately active ulcerative colitis: a randomized study. N Engl J Med 1987;317:1625–1629.Article PubMed
15. Geboes K, Riddell R, Ost A, Jensfelt B, Persson T, Löfberg R. A reproducible grading scale for histological assessment of inflammation in ulcerative colitis. Guta 2000;47:404–409.Article PubMed PMC
16. Nishio M, Hirooka K, Doi Y. Chinese herbal drug natural indigo may cause pulmonary artery hypertension. Eur Heart J 2016;37:1992.Article PubMed
17. Satsangi J, Silverberg MS, Vermeire S, Colombel JF. The Montreal classification of inflammatory bowel disease: controversies, consensus, and implications. Gut 2006;55:749–753.Article PubMed PMC
18. Hirano A, Umeno J, Okamoto Y, et al. Comparison of the microbial community structure between inflamed and non-inflamed sites in patients with ulcerative colitis. J Gastroenterol Hepatol 2018;33:1590–1597.Article PDF
19. Caporaso JG, Lauber CL, Walters WA, et al. Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J 2012;6:1621–1624.Article PubMed PMC PDF
20. Caporaso JG, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods 2010;7:335–336.Article PubMed PMC PDF
21. Kondo S, Araki T, Okita Y, et al. Colitis with wall thickening and edematous changes during oral administration of the powdered form of Qing-Dai in patients with ulcerative colitis: a report of two cases. Clin J Gastroenterol 2018;11:268–272.Article PubMed PDF
22. Yanai S, Nakamura S, Matsumoto T. Indigo naturalis-induced colitis. Dig Endosc 2018;30:791.Article PubMed PDF
23. Matsuno Y, Hirano A, Esaki M. Possible association of phlebitisinduced colitis with indigo naturalis. Gastroenterology 2018;155:576–577.Article PubMed
24. Kim HA, Suh HR, Kang B, Choe BH, Crohn’s and Colitis Association in Daegu-Gyeongbuk (CCAiD). Acute pancreatitis associated with indigo naturalis in pediatric severe Crohn’s disease. Intest Res 2019;17:144–148.Article PubMed PMC PDF
25. Naganuma M, Sugimoto S, Suzuki H, et al. Adverse events in patients with ulcerative colitis treated with indigo naturalis: a Japanese nationwide survey. J Gastroenterol 2019;54:891–896.Article PubMed PDF
26. Nishio M, Hirooka K, Doi Y. Pulmonary arterial hypertension associated with the Chinese herb indigo naturalis for ulcerative colitis: it may be reversible. Gastroenterology 2018;155:577–578.Article PubMed
27. Sato K, Ohira H, Horinouchi T, et al. Chinese herbal medicine Qing-Dai-induced pulmonary arterial hypertension in a patient with ulcerative colitis: a case report and experimental investigation. Respir Med Case Rep 2019;26:265–269.Article PubMed PMC
28. Su C. Outcomes of placebo therapy in inflammatory bowel disease. Inflamm Bowel Dis 2006;12:328–333.Article PubMed

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One-year clinical efficacy and safety of indigo naturalis for active ulcerative colitis: a real-world prospective study

Intest Res. 2022;20(2):260-268. Published online April 29, 2022

DOI: https://doi.org/10.5217/ir.2021.00124

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One-year clinical efficacy and safety of indigo naturalis for active ulcerative colitis: a real-world prospective study

Fig. 1. The clinical response rate and clinical remission rate at weeks 4, 8, and 52 after initiation of indigo naturalis were calculated using non-responder imputation.

Fig. 2. Indicates the week in which the patients achieved a new clinical remission. Thirty patients achieved clinical remission. Although most patients respond early to indigo naturalis treatment, some had delayed response.

Fig. 3. Trends for clinical activity index (CAI) and C-reactive protein (CRP) scores. (A) CAI scores at weeks 0, 4, 8, and 52 after the initiation of indigo naturalis (IN). (B) CRP levels at weeks 0, 4, and 52 after the initiation of IN.

Fig. 4. (A) The mucosal healing rate and the complete mucosal healing rate at weeks 4 and 52 were calculated using non-responder imputation. Histological healing was defined as Geboes score ≤1. (B) Mayo endoscopic subscores at weeks 0, 4, and 52 after the initiation of indigo naturalis (IN). (C) Geboes scores at weeks 0, 4, and 8 after the initiation of IN.

Fig. 5. (A) Alpha diversity by the observed species index and (B) beta diversity by the metrics of weighted UniFrac were not significantly different before and after indigo naturalis treatment. The lines in the box are median and whiskers are interquartile range.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

One-year clinical efficacy and safety of indigo naturalis for active ulcerative colitis: a real-world prospective study

Characteristics	Value (n = 33)
Age (yr)	29 (23–48)
Male sex	18 (53)
Disease duration (yr)	3 (1–8)
Extent of the disease
E2 (left-sided UC)	5 (15)
E3 (extensive UC)	28 (85)
Concurrent medications
Prednisolone	14 (42)
Dose of prednisolone (mg)	6.3 (5.0–18.8)
Anti-TNF-α agents	12 (36)
Cyclosporine	1 (3)
5-ASA	26 (79)
Thiopurine	18 (55)
CAI score	8 (8–11)
MES	2 (2–3)
CRP (mg/L)	3.0 (1.4–8.9)

Adverse events	No. (%)	Duration after initiation of indigo naturalis (day)
Mild adverse events
Headache	8 (24)	1–14
Liver dysfunction	5 (15)	14–41
Mild epigastric pain/abdominal pain	5 (15)	1–28
Renal dysfunction	1 (3)	203
Severe adverse events
Acute colitis	3 (9)	25–258
Infectious colitis	1 (3)	49
Intussusception	1 (3)	25
Appendicitis	1 (3)	270
Portal vein thrombosis	1 (3)	55

Table 1. Clinical Characteristics of UC Patients

Values are presented as median (interquartile range) or number (%).

UC, ulcerative colitis; TNF, tumor necrosis factor; 5-ASA, 5-aminosalicylic acid; CAI, clinical activity index (Rachmilewitz index); MES, Mayo endoscopic subscore; CRP, C-reactive protein.

Table 2. Summary of Adverse Events