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Intest Res > Volume 11(4); 2013 > Article
Intestinal Research 2013;11(4):261-267.
DOI: https://doi.org/10.5217/ir.2013.11.4.261    Published online October 30, 2013.
Epigallocatechin-3-gallate Inhibits the Expression of Adhesion Molecules by Blocking Nuclear Factor Kappa B Signaling in Intestinal Epithelial Cells
Dae-Seong Myung, Young-Lan Park, So-Young Joo, Eun Myung, Cho-Yun Chung, Hyung-Chul Park, Jong-Sun Kim, Sung-Bum Cho, Wan-Sik Lee, Hyun-Soo Kim, Young-Eun Joo
Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea
Abstract
Background/Aims
Epigallocatechin-3-gallate (EGCG) is the main polyphenol in green tea and has anti-inflammatory and anti-oxidative effects. The aim of this study was to determine the impact of EGCG on the expression of adhesion molecules and lipopolysaccharide (LPS)-induced nuclear factor-kappa B (NF-ՊB) signaling in rat intestinal epithelial (RIE) cells. Methods: The effect of EGCG on LPS-induced NF-ՊB signaling and expression of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 was examined by reverse transcription polymerase chain reaction, western blotting, immunofluorescence and electrophoretic mobility shift assay. Results: LPS-induced expression of ICAM-1 and VCAM-1 mRNA was inhibited by EGCG treatment in RIE cells. LPS-induced inhibitor of kappa B alpha degradation and NF-ՊB nuclear translocation were blocked by EGCG in RIE cells. EGCG blocked LPS-induced NF-ՊB DNA-binding activity in RIE cells. The pharmacological NF-ՊB inhibitor Bay11-7082 suppressed the LPS-induced expression of ICAM-1 and VCAM-1 mRNA in RIE cells. Conclusions: These results indicate that EGCG inhibits LPS-induced ICAM-1 and VCAM-1 expression by blocking NF-ՊB signaling in intestinal epithelial cells. (Intest Res 2013;11:261-267)
Key Words: Epigallocatechin gallate, Nuclear factor kappa B, Adhesion molecule, Intestinal epithelial cell
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