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Intest Res > Volume 10(4); 2012 > Article
Intestinal Research 2012;10(4):357-364.
DOI: https://doi.org/10.5217/ir.2012.10.4.357    Published online October 31, 2012.
Synergistic Effect of Parthenolide in Combination with 5-Fluorouracil in SW480 Cells
Se-Lim Kim, Thu Trang Thi Kieu , Byung Jun Jeon, Seong Hun Kim, In Hee Kim, Seung Ok Lee, Soo Teik Lee, Sang Wook Kim
Department of Internal Medicine, Biomedical Research Institute of Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Department of Internal Medicine, Gunsan Medical Center, Gunsan, Korea
Parthenolide와 5-fluorouracil 병용처리에 의한 SW480 대장암 세포의 사멸증진 효과에 관한 연구
김세림, 교티투장, 전병준, 김성훈, 김인희, 이승옥, 이수택, 김상욱
전북대학교 의학전문대학원 내과학교실 임상연구소, 군산의료원 내과
Abstract
Background/Aims
Parthenolide (PT) is responsible for the bioactivities of Feverfew. Besides its potent anti-inflammatory effect, this compound has recently been reported to induce apoptosis in cancer cells. Unfortunately, many of the therapies that use 5-fluorouracil (5-FU) alone or in combination with other agents are likely to become ineffective due to drug resistance. In the present study, we investigate the antitumor effect of PT combined with 5-FU on colorectal cancer cells. Methods: SW480 cell was employed as a representative of human colorectal carcinoma (CRC) cells. We performed MTT, annexin-V assay, and Hoechst 33258 staining to measure the synergistic effect. Western blotting was used to demonstrate apoptotic pathway. Results: Our result demonstrated that PT inhibited the viability of colorectal cancer cells and had synergistic anti-proliferation in combination with 5-FU. After combined treatment of 5-FU and PT, enhanced apoptotic cell death is observed using annexin-V FITC assay and it was revealed by the condensed chromatin and fragmented DNA. Compared with 5-FU or PT alone, the apoptosis of colorectal cancer cells treated with PT and 5-FU enhanced the activation of caspase-8, caspase-3. Conclusions: Combined treatment with PT may offer an efficacious strategy to overcome 5-FU resistance in certain CRC cells. (Intest Res 2012;10:357-364)
Key Words: Parthenolide, 5-Fluorouracil, Apoptosis, Colorectal Neoplasms
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