Synergistic Effect of Parthenolide in Combination with 5-Fluorouracil in SW480 Cells |
Se-Lim Kim, Thu Trang Thi Kieu , Byung Jun Jeon, Seong Hun Kim, In Hee Kim, Seung Ok Lee, Soo Teik Lee, Sang Wook Kim |
Department of Internal Medicine, Biomedical Research Institute of Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Department of Internal Medicine, Gunsan Medical Center, Gunsan, Korea
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Parthenolide와 5-fluorouracil 병용처리에 의한 SW480 대장암 세포의 사멸증진 효과에 관한 연구 |
김세림, 교티투장, 전병준, 김성훈, 김인희, 이승옥, 이수택, 김상욱 |
전북대학교 의학전문대학원 내과학교실 임상연구소, 군산의료원 내과 |
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Abstract |
Background/Aims Parthenolide (PT) is responsible for the bioactivities of Feverfew. Besides its potent anti-inflammatory effect, this compound has recently been reported to induce apoptosis in cancer cells. Unfortunately, many of the therapies that use 5-fluorouracil (5-FU) alone or in combination with other agents are likely to become ineffective due to drug resistance. In the present study, we investigate the antitumor effect of PT combined with 5-FU on colorectal cancer cells. Methods: SW480 cell was employed as a representative of human colorectal carcinoma (CRC) cells. We performed MTT, annexin-V assay, and Hoechst 33258 staining to measure the synergistic effect. Western blotting was used to demonstrate apoptotic pathway. Results: Our result demonstrated that PT inhibited the viability of colorectal cancer cells and had synergistic anti-proliferation in combination with 5-FU. After combined treatment of 5-FU and PT, enhanced apoptotic cell death is observed using annexin-V FITC assay and it was revealed by the condensed chromatin and fragmented DNA. Compared with 5-FU or PT alone, the apoptosis of colorectal cancer cells treated with PT and 5-FU enhanced the activation of caspase-8, caspase-3. Conclusions: Combined treatment with PT may offer an efficacious strategy to overcome 5-FU resistance in certain CRC cells. (Intest Res 2012;10:357-364) |
Key Words:
Parthenolide, 5-Fluorouracil, Apoptosis, Colorectal Neoplasms |
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