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Intestinal Research 2006;4(1):7-11.
Published online June 30, 2006.
Calreticulin Expression in Colon Cancer and Adenoma with Western Blot Assay
Bora Keum, Yoon Tae Jeen, Sanghoon Park, Yeon Seok Seo, Yong Sik Kim, Hoon Jai Chun, Hong Sik Lee, Soon Ho Um, Sang Woo Lee, Jai Hyun Choi, Chang Duck Kim, Ho Sang Ryu
Department of Internal Medicine, Institute of Digestive Disease and Nutrition, Korea University College of Medicine, Seoul, Korea
Western Blot을 이용한 대장암과 대장선종의 Calreticulin 단백 발현 연구
금보라, 진윤태, 박상훈, 서연석, 김용식, 전훈재, 이홍식, 엄순호, 이상우, 최재현, 김창덕, 류호상
고려대학교 의과대학 내과학교실, 소화기연구소
Abstract
Background/Aims
Colon cancer has a multistep process of molecular changes on the way of carcinogenesis. The detection of gene mutations associated with those molecular changes is expected to be able to predict or diagnose colon cancer. Calreticulin is one of the protein which has being investigated as a biomarker of colorectal cancer. The Aims of this study was to demonstrate the expression of calreticulin in normal colon mucosa, colonic tubular adenoma and colorectal cancer of each patient, and analyze the differences among them. Methods: 8 patients who have colorectal adenocarcinoma and more than 10 mm sized tubular adenoma synchronously were enrolled. Colorectal adenocarcinoma, tubular adenoma and normal colon tissue were taken from each patient. After proteins isolation and separation, western blot assay were performed to demonstrate calreticulin. The results were analyzed by Wilcoxon Signed Rank Test. Results: Calreticulin was more expressed in colorectal adenocarcinoma and tubular adenoma significantly compared with normal mucosa. Also, calreticulin was more expressed in adenocarcinoma than in adenoma but there were no significant diffences. Conclusions: These results suggest that calreticulin may be available for an early diagnostic biomarker for colorectal cancer or advanced colon adenoma. (Intestinal Research 2006;4:7-11)
Key Words: Colorectal cancer, Colon adenoma, Calreticulin
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