| BioflorⰒ Suppresses Expression of Interleukin-8 in HT-29 cell |
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Sang Kil Lee, Hyo Jong Kim, Sung Gil Chi, Jae Young Jang, Ki Deok Nam, Nam Hoon Kim, Kwang Ro Joo, Seok Ho Dong, Byung Ho Kim, Young Woon Chang, Joung Il Lee, Rin Chang |
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Department of Internal Medicine, Kyung Hee University College of Medicine, Department of Internal Medicine and Pathology, Kyung Hee University College of Medicine, Seoul, Korea
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| 비오플Ⱂ에 의한 사이토카인 억제에 관한 연구 |
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이상길, 김효종, 지성길, 장재영, 남기덕, 김남훈, 주광로, 동석호, 김병호, 장영운, 이정일, 장린 |
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경희대학교 의과대학 내과학교실, 병리학교실 |
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| Abstract |
Background/Aims
Nowadays, there is a growing interest in probiotics as a safe way of changing the intestinal bacterial flora. Probiotics may have potential in several gastroenterological conditions. The nonpathogenic yeast, Saccharomyces boulardii (S. boulardii, BioflorⰒ), has been used empirically in the treatment of acute infectious diarrhea and antibiotics-associated diarrhea. Recently, S. boulardii was reported to be beneficial in the treatment of inflammatory bowel disease, however, little is known about their mechanisms of action. We examined the ability of S. boulardii to modulate inflammatory response in human colon cells. Methods: Effects of BioflorⰒ on survival and proliferation of HT-29 human colon cells were assessed by MTT and [3H]thymidine incorporation assays. Interleukin-8 (IL-8) expression was stimulated with tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), or lipopolysacharide (LPS). IL-8 was assessed by RT-PCR. Results: BioflorⰒ did not affect viability and proliferation of HT-29 cell. Pretreatment of HT-29 cells with BioflorⰒ blocked IL-8 up-regulation by TNF-α, IL-1β, or LPS to these proinflammatory factors. Conclusions: BioflorⰒ reduces responses to proinflammatory cytokines in human colon cells. (Intestinal Research 2004;2:96-101) |
| Key Words:
BioflorⰒ, , Saccharomyces boulardii, IL-8, HT-29 |
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