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Intestinal Research 2004;2(1):15-20.
Published online April 16, 2004.
Clinical Usefulness of Telomerase and hTERT for the Detection of Colon Cancer in Ulcerative Colitis
Kyu-Jong Kim, Seung-Jae Myung, Seong Soo Hong, Sun-Mi Lee Lee, Jeong-Sik Byeon, Suk-Kyun Yang, Weon-Seon Hong, Jin-Ho Kim, Young Il Min
Division of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea
궤양성 대장염에서 대장암의 조기진단을 위한 Telomerase 및 hTERT의 임상적 유용성
김규종, 명승재, 홍성수, 이선미, 변정식, 양석균, 홍원선, 김진호, 민영일
울산대학교 의과대학 서울아산병원 소화기내과
Abstract
Background/Aims
Telomerase and telomerase reverse transcriptase (hTERT), are specifically expressed in cancer cells, making them candidate markers for the early detection of cancer. The aims of our study were to determine whether these assays may be useful in the diagnosis of colorectal cancer (CRC) developed in ulcerative colitis (UC) patients. Methods: Luminal washings and biopsies were collected during colonoscopy in 66 patients; 34 with CRC, 21 with UC, and 11 controls. Telomerase activity was detected by telomeric repeat amplification protocol (TRAP) and hTERT was assayed by RT-PCR. Results: Telomerase activity was detected in biopsies from 33/34 (97%) CRC, 14/21 (67%) UC, and 3/11 (27%) controls. hTERT was positive in biopsies from 32/34 (94%) CRC, 12/21 (57%) UC, and 5/11 (45%) controls. In washing fluid, 21/34 (62%) of CRC patients were positive for telomerase, but UC patients and controls were negative. The sensitivity of telomerase for CRC was 97% in tissues and 62% in washing fluid. The specificity of telomerase in washing fluid was 100%, whereas the specificity of telomerase or hTERT mRNA in tissues was 47% each. Conclusions: The low specificity of telomerase and hTERT in colonic tissue suggest that these are not candidate markers for CRC arising in UC. Telomerase in colonoscopic luminal washings, however, may be a novel marker for early CRC in UC. (Intestinal Research 2004;2:15-20)
Key Words: Colorectal cancer, Ulcerative colitis, Telomerase, hTERT, Colonoscopy
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